Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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Lab2: Testing for Proteins and Starches In this lab 8 total substances were tested to find out whether they are a Protein or a Starch. It is my belief that only 1 or two of each of the substances in test 1( proteins) and test 2 (starch) will test positive for either protein or starch. For this lab the following materials were needed to complete the experiments in test 1 for proteins: Di water‚ ev milk‚ 50% egg solution‚ 1% sucrose‚ 4 test tubes‚ 1 test tube rate‚ safety glasses‚ pipets and
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Starch/Amylase Experiment Report Objective: The purpose of the starch/amylase experiment was to simulate and observe the process of enzyme digestion. Materials: * 1 small beaker * 2 large beakers * 2 cut pieces of soaked dialysis tubing * 2 dialysis tubing clamps or pieces of twine * 2 clean plastic pipettes * 1 bottle of Lugol’s solution * 2 glucose test strips Procedure: Begin the experiment by placing 4 full pipettes worth of cooked starch in a beaker. Then‚ use
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Title: Benedict ’s test for Reducing Sugars Testable Question: Are all carbohydrates reducing sugars? Hypothesis: If the carbohydrate contains available ketone or aldehyde molecules then they are reducing sugars. Variables: a. Independent: Function groups react w/ reagent b. Dependent: Color change (red‚ orange‚ yellow) Control: Water Materials: - 4 mL of each solution (Water‚ Starch‚ Glucose‚ Maltose‚ Sucrose‚ Onion Juice‚ Potato slice‚ Milk) - 8 test tubes
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substances which do not interact with water are called hydrophobic Water as an universal solvent Ions and polar molecules are charged. They are attracted to water molecules because of the weak positive and negative poles and are therefore dispersed easily in water forming solution Water is an universal solvent (due to its high polarity) for numerous of biochemical molecules‚ giving solution and enabling • Transport of nutrients • Secretion of substances Water in metabolic reactions
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Daniel Bergey Lab 2: Proteins and Starches Purpose The purpose of lab 2 and both tests with proteins and starches is to determine which substance contains either protein or starch. Hypothesis Proteins: I predict that any substance I test that derives from a living organism is will test positive proteins. Any substance that isn’t from a living organism more than likely will test negative for proteins. Starches: I predict that any substance that contains any level of glucose will test positive
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Simple Sugars Lab Emerson: Hallie: Background: Benedicts solution is a chemical solution that changes color in the presence of glucose and other reducing sugars. It is a mixture of sodium or potassium citrate‚ sodium carbonate‚ and copper sulfate. It changes from its normal blue color to an orange‚ red‚ or yellow. it changes based on the amount of sugar used. "Benedicts Solution." Definition from BiologyOnline.org. N.p.‚ n.d. Web. 16 Jan. 2015. http://www.biologyonline.org/dictionary/Benedicts_solution
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how starch and cellulose are treated to allow them to be used by the yeast? One potential ethanol feedstock is starch. Starch molecules are made up of long chains of glucose molecules. Thus‚ starchy materials can also be fermented after breaking starch molecules into simple glucose molecules. Examples of starchy materials commonly used around the world for ethanol production include cereal grains‚ potato‚ sweet potato‚ and cassava. A great amount of ethanol fuel is currently produced by starch
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Brittney Humphries. Title: Testing for the Presence of Starches‚ and Proteins Purpose: To test for the presence of starches and protein macromolecules‚ to introduce the concept of variations in chemical compounds. Hypothesis: If biuret is blue after a test for protein‚ then it is a negative result because biuret reagent is blue to begin with. If a solution is pinkish purple‚ or purple‚ then the test for protein is positive. The test solution for starches is yellowish brown. If any substance
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Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify
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