Expressing and Purifying the Recombinant form of Green Fluorescent Protein (rGFP) from the E.coli strain using Ni2+ agarose affinity chromatography technology Abstract The purpose of this experiment was to express and purify the his6-tagged recombinant form of GFP (rGFP) from the organism E.coli using Ni2+ agarose affinity chromatography. The expression of rGFP was confirmed qualitatively using the UV light and was expressed in the E.coli strain BL21 (DE3) (-- removed HTML --) (-- removed HTML
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3.1 Proteins Table 3.2 Biuret Test Tube Contents Final Color Conclusions 1 Distilled water Transparent‚ light blue‚ navy Possibly little protein with clear peptide or no protein at all 2 Albumin Dark Purple Proteins are present with purple peptides 3 Pepsin Purplish blue‚ darkish blue Proteins are present with purple or black colored peptides 4 Starch Light blue‚ really clear Possible little protein with clear peptide or no protein at all Our results are correct because water
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Protein in the Digestive System Taylor Adams Biol 112- 501 18 April 2016 Introduction Proteins are found in nearly all foods that we eat. Once the food we eat makes its way to our stomachs‚ pepsinogen is released from chief cells. This enzyme mixes with hydrochloric acid in the stomach and begins to break down the proteins. Along with the stomach‚ the small intestine is also an important location for protein breakdown. The proteins from both locations are broken down into amino
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Abstract The objective of this lab was to measure the amount of protein from a piece of beef liver . This was done by taking the liver‚ blending it and then using a centrifuge to separate the supernatant from the pellet. Once that was completed‚ ammonium sulfate was added to the supernatant‚ chilled and then spun for a second time. Next‚ 20 mL of water is added to the pellet‚ stirred and the volume was recorded. The teacher calculated the total mass of liver to be 10.098g. Lastly a spectronic
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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This is a documentary released by BBC titled “Sugar VS Fat”‚ starred by Alexander Van Tulleken and Chris Van Tulleken. It is obvious that this is a program about the disadvantage and benefit of sugar as compared to fat. Yet‚ one should not judge the book by its cover‚ for the highlight of this documentary is the deadly combination of processed food with a ratio of 1:1 fat and sugar in it. Obesity is an anciently known problem. Over hundreds of years‚ scientists have estimated some possible cause
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BIOLOGY LABORATORY Biologically Important Molecules Carbohydrates‚ Proteins‚ Lipids‚ and Nucleic Acids Objectives In this lab you will learn to: 1. Perform tests to detect the presence of carbohydrates‚ lipids‚ proteins‚ and nucleic acids 2. Recognize the importance of a control in a biochemical test 3. Use biochemical tests to identify an unknown compound Introduction Organic molecules are those primarily made up of carbon‚ hydrogen and oxygen. The common organic compounds of living
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Specificity of Protein-Ligand Binding 1). One can conclude that Orange G. has the lowest affinity for the albumin. In the experiment the concentration of Orange G that binds to protein was a lot less than either Ponceau S. or Bromophenol blue. The ligand with the highest affinity to BSA was a little more difficult to decipher. The experiment shows that all of the 2 µL of both Ponceau S. and Bromophenol blue bind to the BSA. However‚ when 5 µL of the ligand is added the BSA is saturated and cannot
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Determining the sugar concentration of food samples is very important especially in industries where quality control is monitored. One method to determine the sugar concentration of reducing sugars is by heating with 3‚5 dinitrosalicylic acid(DNS) which produce a red-brown product Miller(1959)The reaction is direct‚thus the method is preferred over the Benedict’s test method. The concentration of the coloured complex can be determined with the spectrophotometer at Absorbance 540.The sugar concentration
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brown sugar on the amount of CO2. To do this‚ white sugar was replaced with brown sugar and the amount of CO2 produced was calculated with a vernier gas pressure sensor. The control group was yeast and white sugar and the experimental group was yeast and brown sugar. The hypothesis that if brown sugar and yeast are mixed‚ then it will produce more CO2 than a mixture of white granulated sugar and yeast was accepted. The white sugar and yeast mixture had a slope of .003254 kPa/s. The brown sugar and
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