BSC 2010C.LAB TH 7-9:50pm 29 August 2013 Biology Lab Report Lab #1 –PROTEIN EXTRACTION LAB I. INTRODUCTION To begin the process of protein extraction and compare the results in a study‚ it is necessary to understand the importance of proteins‚ the process of extraction and how you are using the results to determine a rational conclusion. First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the
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Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according to size using an electric current. Proteins that are separated after are blotted from the gel and onto a paper membrane. An antibody is then added to the membrane paper and causes a colored reaction. Following the reaction‚ the results help detect and quantify a single
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bind to a specific antibody‚ the SDS-PAGE and Western Blot was performed. SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE) is a very common technique used to separate proteins based on molecular weight under the influence of an applied electrical field and then used to prepare for the Western Blot (#1 Lehninger). The support medium used is a polyacrylamide gel and‚ it also used sodium
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The Isolation‚ purification and identification of Proteins assayed From Bovine Liver Using SDS Gel Electrophoresis‚ Mass Spectroscopy and Western Blotting Abstract The purpose of the experiment was to isolate and recognize varying protein solubilization and assaying methods by using bovine liver protein. The experiment implicated the impact of different types of solvents like ethanol‚ water‚ PBS‚ PBS+1% Triton x-100‚ and PBS+2% SDS on protein solubilization. Bradford and Ghosh/Dumbroff
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was designed to use western blot analysis to detect proteins from Non-fat powdered milk. The experiment had to be run two times before the expected outcome was reached. However‚ after the second attempt‚ it was successful. The gel ran well‚ and the proteins from the gel were successfully transferred to the membrane provided in the kit. Introduction The name western blot was given to the technique by W. Neal Burnette and is a pun on the name Southern blot. Southern blot was a name given by
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Ostry‚ Laichmannova‚ Ruprich‚ 2010). Enrico Dainese and his partners did another similar study‚ on soybeans specifically. Like our experiment conducted on the cornbread mix‚ Dainese and his colleagues followed their PCR results with an Agarose Gel Electrophoresis (Dainese‚ Angelucci‚ De Santis‚ Maccarrone and Cozzani‚ 2004). An additional experiment closely related to the one performed by my partners and I is a study done in Brazil to better detect for GMO within their foods sold in markets a other places
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agarose gel electrophoresis in an emulation of DNA fingerprinting. The task‚ which was successfully carried out was to determine whether DNA from suspects A‚ B or C matches the sample of blood found at the murder scene (X). The process of PCR acts in the same way as DNA replication but is restricted to specific DNA samples of interest. By amplifying the necessary DNA sequence‚ this procedure is able to produce a usable DNA sample for agarose gel electrophoresis. Agarose gel electrophoresis is a method
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Lab Report Electrophoresis: Using Electricity to Separate Molecules Answer the following questions about the results of this activity. Record your answers in the boxes. Send your completed lab report to your instructor. Don’t forget to save your lab report to your computer! Activity 1 – Calibration Record your data from Activity 1 in the boxes below. Enter the data (number of bands‚ description of the band patterns) you collected for the protein ladder in the appropriate columns
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2.6 Kinetic studies of prepared complexes The integral method of Coats–Redfern equation[19‚21‚27‚38] was used for determining the kinetic parameters of the decompositions process for the investigated metal complexes according to following equation: log[log(w_∞/(w_∞-w))⁄T^2 ]〖=log[AR/〖∅E〗^* (1-2RT⁄E^≠ )]〗-E^≠/2.303R 1/T (4) Where w_∞ is the mass loss at the accomplishment of the decomposition reaction‚ w is the mass loss at temperature T‚ ∅ is the rate of heating and R is
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