plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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DNA STRUCTURE AND REPLICATION One may wonder how a single cell becomes two cells‚ and why this is ultimately important to life. For this essay‚ it would be much too difficult to discuss the process of cell division‚ which is the biological basis of life. That being said‚ this essay will examine more closely the precursor to cell division‚ also known as DNA replication. DNA replication is the process of copying a double-stranded DNA strand on a chromosome within a cell. The process‚ in its totality
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company name] | DNA Research Paper | Coach Jones 6th period | | Austin Permann | 4/11/2011 | | Austin Permann 4/11/11 Period 6 Jones My DNA Research Paper DNA is something that is found in almost every organism. It is an abbreviation for deoxyribonucleic acid. It is the hereditary material in people and is located in the cell nucleus. DNA contains the genetic instructions used in the development and functioning of all known living organisms. DNA is pretty much
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The DNA double helix model In 1953 James Watson and Francis Crick made a scientific breakthrough when they discovered the structure of DNA. From this structure they inferred that DNA contains information written in a genetic code‚ is passed from one generation to the next. This discovery was to be a key step in the genetics revolution DNA has four molecular building blocks called nucleotides; adenine (A)‚ thymine (T) guanine (G) and cytosine (C). Purine base always pairs (by hydrogen bonding) with
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EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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Extracting DNA from Wheat Germ Cells Criteria to be assessed CE Introduction: DNA is the abbreviation for deoxyribonucleic acid. DNA is found in the nucleus of every cell & it stores the information that makes up living organisms. It is a double helix of 4 nucleotides. It contains a code that allows the body to make up proteins. The nucleotides are Cytosine‚ Guanine‚ Adenine and Thymine. (1) Wheat germ comes from wheat seeds. The ’ germ ’ is the embryo‚ which is the part of
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Homework # 1 DNA 2. Name the four differences between RNA and DNA. 1) DNA is double stranded‚ RNA is single 2) DNA has Adenine‚ Guanine‚ Thiame‚ and Cytosine as nitro bases. With RNA‚ anywhere you would expect to see Thaime‚ you see Uracil instead. 3) DNA has no types‚ RNA has 3 types: mRNA‚ rRNA‚ and tRNA. 4) DNA and RNA have different sugars. 3. For the following DNS strand‚ provide the replicated strand : GATTACACATTAG CTAATGTGTAATC 4. For the following DNA sequence provide
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Deoxyribonucleic Acid (DNA) DNA stands for deoxyribonucleic acid‚ it is located in the nuclei of cells which make up the body. DNA is quite often referred to as one of the building blocks of the body.. It is made up of four bases known as: • Adenine • Guanine • Cytosine • Thymine James Watson‚ Francis Crick‚ Maurice Wilkins‚ Rosalind Franklin Crick and Watson‚ together with Maurice Wilkins‚ won the 1962 Nobel Prize in physiology or medicine for their 1953 determination of the structure of
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The purpose of the DNA extraction lab was not only to inform students on how DNA is present in humans and all organisms‚ but to also educate them on how DNA can be extracted using common household materials. Also‚ the lab was very efficient as it introduced the students to extracting their own DNA found on their cheek cells as well as letting them take an observation on how DNA appears or how it is formed. Additionally‚ students were instructed through a very clear and simple procedure‚ which guided
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the structure of DNA. ➢ DNA is a nucleic acid‚ which consist of long chains (polymers) of chemical units (monomers) called nucleotide. A molecule of DNA contains two polynucleotides‚ each a chain of nucleotides. Each nucleotide consists of a nitrogenous base‚ a sugar‚ and a phosphate group. Each DNA strand serves as a mold‚ or template‚ to guide reproduction of the other strand. There are four different types of nucleotides found in DNA‚ differing only in the nitrogenous base. DNA is contained in
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