Biology investigation: Liver and hydrogen peroxide By Cuong Tran Abstract: The purpose of this investigation was to determine the optimum temperature for enzyme activity. The stated hypothesis was that 37oC would be the optimum temperature for liver enzymes to break down hydrogen peroxide into water and oxygen. The dependent variable was to measure the amount of foam being produced‚ while testing 4 different temperatures with 3 repetitions each. Out of the 12 trials only 2 outliers were found
Premium Oxygen Hydrogen peroxide Enzyme
temperature‚ pH and concentration. Catalase is one of the most common enzymes. It is found in living organisms and is used to break down hydrogen peroxide. This must happen because hydrogen peroxide is considered toxic to cells in the body. However‚ when catalase is used it breaks it down into hydrogen and oxygen‚ which is much safer to have in the body. A substrate is the material that is being broken down. In this case‚ the substrate would be hydrogen peroxide. The catalase‚ located in the liver‚ is being
Premium Chemical reaction Hydrogen peroxide Water
concentration‚ temperature and pH on the functionality of the enzyme catalase. The enzyme lab was also about measuring reactions by capturing the oxygen that was generated by the reaction. Materials and Methods: Experiment 1‚ 2‚ & 3 Experiment 1 examined the effects of concentration on catalase activity. Experiment 2 examined the effects of concentration in temperature on catalase activity Experiment 3 examined the effects of pH on catalase activity The eudiometer was filled with water and inserted
Premium Catalase Enzyme Oxygen
Activity Abstract Our experiment looked at how increasing the surface area of a substance affects the amount of bubbles created due to the presence of the enzyme catalase. The experiment used two pieces of fish‚ one whole and one ground up‚ which were then covered in hydrogen peroxide. This method allowed us to observe the catalase in ground up fish break down the hydrogen peroxide at a quicker rate than in the piece of fish left intact. This was determined by observing a larger formation of
Premium Catalase Chemical reaction Hydrogen peroxide
Title: The effect of the concentration of hydrogen peroxide on catalase Aim: to examine how the concentration of the hydrogen peroxide affects the rate of reaction Hypothesis: The substrate concentration will determine the rate of reaction. The reaction will increase as the concentration increases‚ but from a certain concentration‚ the rate will maintain the same rate Materials: 3% hydrogen peroxide 6% hydrogen peroxide catalase round metal basin measuring cylinder thermometer conical flask
Premium Catalase Enzyme Concentration
To determine the effects of pH on catalase enzyme activity.Enzyme catalase Lab Statement of the problem : Catalase breaks down H2O2 (hydrogen peroxide) into water and oxygen. H2O2 is a toxic substance formed during metabolism. Hypothesis: To determine the effects of pH on catalase enzyme activity and as you increase the pH the more oxygen that will be produced. Materials: 1. 5 clean test tubes 2. HCI (acid) 3. Dilute HCI 4. Water 5. NaOH (base) Procedure: We start to fill
Premium Catalase Enzyme Oxygen
llllllllllllllllllllllllllllllllllllllllllllllllllldfdkf Determine the presence of catalase enzyme activity using hydrogen peroxide. 1. Test for the presence of catalase in liver. A. Using a knife‚ cut a slice of liver that measures approximately 1 cm by 1 cm and place it on a slide. B. Using a micropipette‚ place a few drops of hydrogen peroxide (H2O2) on top of the liver. C. Observe the solution for a possible reaction‚ indicated by bubbling. Record the results. 2. Test for the presence of catalase in lunch meat. A. Cut a piece of roast
Premium Catalase Enzyme Hydrogen peroxide
concentration H2O2 (l) solution affect the amount of oxygen released between 15.0ml of the solution and a 5g sample of liver? The reaction is measured by the difference in mass of a balloon that is used to capture the amount of gas released by the catalase enzyme found in liver reacting with a basified 3% H2O2 (l) and an acidified 3% H2O2 (l) . Chemical reactions occur when two or more molecules interact and the molecules change. Bonds between atoms are broken and recreated to make new molecules
Premium Acid Oxygen Chemistry
toxin by potato’s enzyme catalase‚ determine factors that influence how quickly the reaction takes place‚ and determine factors that influence how well enzymes function. The first test‚ we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added a pinch of sand into the test tube. For the first test tube we observed that the hydrogen peroxide did not react with the sand because there is no catalase in the sand. Our time
Premium Catalysis Catalase Chemical reaction
disks Part A: Observe the Catalase Reaction 1. Put on apron and safety goggles. Use forceps to place a small piece of raw liver in an open petri dish. Use a dropper pipette to put a drop of hydrogen peroxide solution on the liver. CAUTION: Hydrogen peroxide can be irritating to skin and eyes. If you spill any on yourself or your clothes‚ wash it off immediately and tell your teacher. Observe what happens. RECORD in notebook. Liver contains the enzyme catalase‚ which breaks down hydrogen
Premium Enzyme Catalase Catalysis