00000012636 (BM1/14) Title : Spectrophotometer and its function Introduction Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It can measure any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. [1] There are a few types of spectrophotometer such as calorimeter‚ UV spectrometer‚ IR spectrometer‚ atomic spectrometer and mass spectrometer
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ACKNOWLEDGEMENTS It is usually said that no man is an island unto himself‚ and any man who so lives all by himself‚ without interacting with his fellow men is in the words of Aristotle‚ “either a beast or a god”. Mutual co-operation and interaction form the cardinal feature of human life. The task of writing this report has not‚ in the least‚ been an easy one‚ and it would be axiomatic to add that all the numerous tasks involved could not be have been accomplished successfully singly
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Technical Specification Sheet For UV-Spectrophotometer | | Wavelength range | 190 to 1‚100 nm | Spectral bandwidth | 1 nm (190 to 1‚100 nm)/ variable Low stray light < 0.12%T (220 nm‚ NaI 340nm‚ NaNO2>2.0 Abs) | Wavelength accuracy | ± 0.1 nm at D2 peak 656.1 nm‚ | | ± 0.3 nm for entire range | Reproducibility | ±0.1 nm | Resolution | 1 nm more than 6 wave lengths | Photometric system | Double beam optics | Photometric range | Absorbance: -4 to 4 Abs | | Transmittance:
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mind that all the tubes that would enter the spectrophotometer are clean and dry before the substrate and buffer was added. In the first experiment‚ optimal temperature for enzymatic activity was tested. Five clean spectrophotometer tubes wereare necessary with the different temperatures labeled on them using a wax pencil (Blank‚ Room Temp‚ 35 degrees Celsius‚ 45 degrees Celsius‚ and 55 degrees Celsius). Then 1mL of
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Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a & b) are
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Group No. 10 Date Started: June 11‚ 2013 Tongco‚ Val Justin DM. Date Finished: June 18‚ 2013 Santos‚ Erickson S. Vasquez‚ Jona Candace P. Wong‚ Victor Lorenzo E. Experiment 1 Use of Micropipettor and Spectrophotometer Introduction In scientific experiments‚ correct measurements are required to achieve precise and accurate data. Precision is the degree to which several measurements provide answers very close to each other. Accuracy describes the nearness of a measurement to the
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Introduction In order to determine the content of calcium and magnesium in an unknown sample of limestone‚ the utilization of atomic absorption spectrophotometry can help in deciphering the unknown concentrations of each molecule in the limestone sample. Atomic absorption spectrophotometry‚ emits radiation of the correct frequency that is passed through a flame and the intensity of the transmitted radiation is measured¹.The calcium concentration or ppm can be measured using the absorbance of the
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Technical Paper WFGD Case Study -- Maximizing SO2 Removal by Retrofit with Dual Tray Technology A.A. Silva and P.J. Williams The Babcock & Wilcox Company Barberton‚ Ohio‚ U.S.A. J. Balbo Michigan South Central Power Agency Litchfield‚ Michigan‚ U.S.A. Presented to: EPRI-DOE-EPA-AWMA Combined Power Plant Air Pollutant Control Mega Symposium August 28-31‚ 2006 Baltimore‚ Maryland‚ U.S.A. 6 The Babcock & Wilcox Company BR-1785 WFGD Case Study - Maximizing SO2 Removal
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Proteins in Solution by Spectrophotometer Lab bench# 1 Introduction: Absorption spectroscopy is a common method for finding the concentration of proteins or protein complexes in a solution. Proteins absorb light at specific wavelengths and can be defined by the equation A = log (Io/I). This equation states that an absorbance at a specific wavelength‚ A is equal to the log of the ratio of incident light intensity (Io)‚ to transmitted light intensity (I). A spectrophotometer can be used quantitatively
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A removal process consisting of absorption‚ desorption‚ heat exchangers and auxiliary equipment is shown in Figure 10. Absorption is traditionally performed in a column with plates‚ random packing or structured packing. CO2 containing gas flows upwards and the absorption liquid flows downwards. The solvent (rich amine) is pumped further through a heat exchanger to a separation column. The absorbed CO2 is regenerated in a separation (stripper) column. Heat is added to the reboiler and a condenser
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