OPPapers.com

Unknown Lab Report Grading Guidelines

Cover Page (5 points)
____Your name and lab section (2 pts)
____Unknown # and genus and species of unknown correctly spelled and italicized (3 pts)

Gram Stain (20 points)
____Correctly stained smear with individual organisms demonstrated using 100x objective (5 pts)
____Description of what you see in words including Gram stain reaction, cellular shape
and arrangement (5 pts)
____Indicate the type of microscope and total magnification used (5 pts)
____Draw a picture (colored and labeled) of the stain results (5 pts)

Primary Media and Streak Plates (15 pts)
____Isolated colonies on the streak plate (5 pts)
____Written description of isolated colonies on the streak plate (5 pts)
____Discussion of growth on TSA or SBA plate after the initial isolation (5 pts)*
*This will differ for each person – may include hemolytic pattern, level of growth at different temperatures or aerobic vs. anaerobic growth

Biochemical Tests (Use a table format) (20 pts)
____Must include KOH, catalase, oxidase and ALL other tests carried out (5 pts)
____Correctly identify the reagents and/or media used in every test (5 pts)
____Correctly identify the enzyme and/or end products the test is looking for
____Correctly identify and interpret the reaction observed (color change, etc)

Dichotomous Key
____Correctly list the five families of bacteria at the initial Gram stain step (2 pts)
____Follow the appropriate format and separate the five families until each family occurs
individually
____Continue under the correct family until your unknown is keyed out (5 pts)

Discussion
____Describe any problems you encountered or results that were atypical (5 pts)
____Describe how you arrived at the identification of your unknown (5 pts)
____Discuss what you learned, what you would do differently, etc. (5 pts)

Miscellaneous
____Correct spelling of all media, enzymes, reagents, and bacterial names (5 pts)
____Correct scientific notation of...