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Proteus mirabilis. Investigative Journal 10/05 ... working stock. b. I had eliminated
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Submitted by adebolaoyekoya on March 27, 2006
Category: Science
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Investigative Journal
10/05/05
Journal # 1
Today I was given my unknown broth and began my journal.
Unknown broth
a. I received unknown broth #34, a tube of distilled water, and a TSA plate of media.
b. I recorded the number of my unknown, #34, onto my Descriptive Chart.
c. I labeled my TSA plate, my tube of unknown broth, and my distilled water with my name, date, lab section, media name, and my unknown number.
Growth characteristics from broth
d. I observed the broth for growth characteristics. I characterized the surface as membranous because there was a thin film of growth at the top. The subsurface I characterized as turbid because it was very cloudy. After agitating the tube, I was able to characterize the sediment as viscid.
e. I recorded the broth growth characteristics onto my Descriptive Chart. (Brown, pg 226)
i. Surface: Membranous
ii. Subsurface: Turbid
iii. Sediment: Viscid
Four quadrant streak
f. I rolled my broth tube to get a good dispersion of the organism.
g. Next, using four quadrant streak techniques for isolated colonies, I stroked my TSA plate. (method A, Brown, p 73)
h. I labeled, inverted, and stored the TSA plate in the incubator at 37 C for 24-48 hours.
Gram stain and morphological characteristics of my unknown
i. In order to define the morphology and Gram reaction of my unknown, I followed the procedure for making a smear from my unknown (Brown, page 84). I used the aseptic procedure for organism removal, (Brown, page 85) and Gram stained using five loopfuls of my broth (Brown pg 96). I then stored my unknown broth and distilled water in the refrigerator rack.
j. I observed my Gram stained slides with my microscope (Brown pg 6-8) and recorded morphological characteristics (Brown pg 45, 95) of the gram stained broth onto my descriptive chart. I stored the slides...
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