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Photosynthesis lab Introduction To survive, all organisms need energy. Plants get most of their energy from photosynthesis. Plants are autotrophs, that generate
Photosynthesis lab Photosynthesis Lab Data Collection: The absorption of different wavelengths of light by Chlorophyll Wavelength (nm) Absorbance of light by chlorophyll
Chloroplast Lab Determining Rates of Photosynthesis Through Chloroplasts Introduction: 1)Background= 2) Purpose= measure the rate of photosynthesis in chloroplasts.
determine which wavelengths of light can be utilized most effectively in photosynthesis. The purpose of this lab was to help students gain a firmer grasp on how different
Exercise 12 Lab Section 10 TITLE: The Affects of Light on a Geranium Leaf's Starch Accumulation INTRODUCTION: In order for all life forms to survive the circle has
Submitted by irblack on December 18, 2006
Category: Science
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Introduction
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To survive, all organisms need energy. Plants get most of their energy from photosynthesis. Plants are autotrophs, that generate their own chemical energy from the product of the sun through photosynthesis. The chemical energy that plants get from the sun is used to produce glucose that can be burned in mitochondria to make ATP, which is then used to drive all the energy-requiring processes in the plant.
Photosynthesis occurs in plants in the chloroplast, an organelle specific to plant cells. In prokaryotes that perform photosynthesis, there are no chloroplasts; instead, photosynthesis occurs in association with the plasma membrane or infoldings of the membranes. Chloroplasts are found mainly in the cells of mesophyll, the green tissue in the interior of the leaf.
In the lab we replaced the electron-accepting coenzyme, NADP, with the blue DPIP dye. When DPIP was reduced, it had changed its color from blue to colorless. The DPIP reduction led to the decrease in light absorbance measured by the colorimeter.
Procedures
Plant Pigment Chromatography
1. Obtain and wear safety goggles and a lab apron.
2. Obtain a chromatography bottle with 5mL of solvent.
3. Cut the filter paper so that the bottom will touch the bottom of the bottle and still stick out of the top.
4. The normal length is about 15 cm.
5. Cut the end going into the solvent into a point
6. Draw a pencil line about 2 cm above the pointed end of the paper.
7. Use a coin to extract the pigments from the spinach leaf.
8. Place a small section of the leaf on top of the pencil line.
9. Use the ribbed edge of the coin to push the plant cells into the chromatography paper.
10. The more times you do this, the better.
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