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Oral Glucose Tolerance Test Experimental Purpose The purpose of the Variables in Glucose Tolerance lab was to examine the changes in blood sugar during glucose tolerance
Investigating the oral glucose tolearnce test Investigating the oral glucose tolerance test Aim: To carry out glucose tests on stimulated blood plasma samples if
venous glucose concentration > 11.1 mmol / L two hours after taking 75 g glucose in an oral glucose tolerance test (OGTT). There are two types of DM; insulin dependent
level of 126 mg/dl or higher. Another way to prove a diagnosis would to be to have an OGTT or Oral Glucose Tolerance Test that reveals a blood glucose of 200 mg/dl
test for diabetes is now a fasting plasma glucose test rather than the previously preferred oral glucose tolerance test. (However, in certain clinical circumstances,
Submitted by jsmalls1 on July 17, 2008
Category: Science
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Experimental Purpose
The purpose of the Variables in Glucose Tolerance lab was to examine the changes in blood sugar during glucose tolerance test conducted with test rats. We will be using three rats and perform the Oral Glucose Tolerance Test to measure the glucose levels in the rats blood. The oral glucose tolerance test is a laboratory method to check how the body breaks down (metabolizes) blood sugar. Glucose is the sugar that the body uses for energy. Patients with untreated diabetes have high blood glucose levels. Glucose tolerance tests are one of the tools used to diagnose diabetes. (Hypothysis)
Materials ad Methods
The independent project is a group project in which the class in split into groups of 4-5. My group consisted of James, Jaime, Omar, Kirk, and Myself. We selected our two drugs which were Glyberide and Insulin. We discussed who would be doing what on the day of the project and how we would manage our time. I had to record all of the data that we received form our experiment and observe the behavior patterns that rats displayed inside their plastic restraint. Omar was responsible for researching the affects of glucose; James was responsible for researching the affects of insulin, and Kirk and Jamie were responsible for researching the affects of glyberide.
The materials that were used in the independent project were 3 male rats, one touch glucometer and strips, gloves, syringes, scissors, orange marker, plastic rat restraint, and a stop watch to conduct our project. We selected three rats and marked their tails with orange sharpie in order to differentiate between the rats when we conduct the actual project. We weighted each of the rats to ensure we give accurate amounts of each drug. Previous to selecting the rats, the food was removed form his cage for approximately 24 hours so the rats could undergo a fasting cycle. The three drugs we used were Glucose, Insulin, and Glyberide. When determining how much of each...
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