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Submitted by mugbugrulz on February 26, 2007
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Unknown Number 29
Enterobacter aerogenes
TESTS DONE
1. Gram Stain Gram Negative Rod
2. SIM tube motile, non-sulfur producing, indole negative
3. Simmon's Citrate tube positive
4. Durham tubes
a. Does ferment galactose
b. Does not ferment glucose
c. Does ferment maltose
d. Does ferment glycerol
e. Does ferment sucrose
f. Does ferment trehalose
5. Urea tube urease negative
6. Oxidase test does not produce cytochrome oxidase
7. Catalyse test produced bubbles
8. Grew at 41۫C and not at 4۫C
9. Spirit Blue Agar lipase positive
10. Lysine Decarboxylate lysine negative
11. Nitrate Broth nitrites produced (turned red after adding both reagents)
12. MR-VP medium
a. Methyl red positive
b. Indole negative
c. Voges-Proskauer negative
d. Citrate utilization positive
According to Bergey's Manual, the Genus Enterobacter are straight rods, Gram negative. Most are motile and classified as facultatively anaerobic. Their optimal temperature is 30-37۫C, which is different from my results. It did grow at 37۫C, but it also grew at 41۫C. This genus is usually lysine negative and lipase is not produced. I completed addition tests that were selective for enteric bacteria:
1. EMB medium grew uniform purple colonies
2. SS medium grew uniform pink colonies
3. MacConkey's medium grew uniform pink colonies
4. Desoxycolate medium positive reaction (the plate turned yellow)
5. MSA medium positive reaction (the plate turned yellow)
This genus of bacteria are widely distributed in nature, occurring in fresh water, soil, sewage, plants, vegetables, and animal and human feces. They are...
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