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Submitted by Zoupa on March 27, 2008
Category: Science
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Factors Affecting the Rate of Enzyme Activity
Malt amylase (enzyme) was tested in different enzyme concentration, substrate concentration and environmental factors such as pH level, temperature and inhibitor (ZnCl2) in order to see the effect of those factors to the ability of enzyme breaking down substrate. In the experiments for effects of changes in enzyme concentration, substrate concentration and inhibitor on enzyme’s function, one drop of iodine was added to three controls and nine variables and each variables contained different concentration of enzyme, substrate or inhibitor depending on the experiment. In the experiments for effects of changes in temperature and pH on enzyme’s function, one drop of iodine was added to each test tube. The experiments for temperature and pH contained 3 different parts: test tubes with water at room temperature (25℃), 7℃ and 100℃ were tested and test tubes with pH 7, pH 4 and pH 11 were tested. Since this lab was performed for two days, the concentrations of enzymes were different between two days. Although measuring standard time curve for enzyme was performed for both day, the different concentration of enzyme could have brought variations to the results by not having same controls for two days. This might have cause wrong interpretation of results as variables were needed to be compared with controls to interpret the results. This experiment should have been performed in one day so that the same concentration of enzyme could be used to create consistent enzymes to observe the enzyme activity and see results by comparing with consistent controls.
Introduction
Enzymes are functional proteins that catalyze specific reactions in living organisms. It is often referred as biological catalysts because of its ability to help endothermic and exothermic reaction in living...
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