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Does Caffeine affect the heart rate of Daphnia. Does caffeine affect the
heart rate of Daphnia? Introduction In this experiment I ...
AN INVESTIGATION TO SEE THE EFFECT THAT CAFFEINE HAS ON THE HEART BEAT ... looking at
that it is clear to see that alcohol does affect the heart rate of Daphnia ...
Submitted by ac_20 on May 10, 2007
Category: Science
Words: 891 | Pages: 4
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Does caffeine affect the heart rate of Daphnia?
Introduction
In this experiment I aim to find out if caffeine has an affect on heart rate. Instead of using human subject, whom may already have a high tolerance to caffeine or could have ingested caffeine that day making the tests unfair, I will use Daphnia, a crustacean. They have on contact with caffeine making it fair. It will be easier to observe as it has a translucent body, making it able to see the heart beating under a microscope. It will be a "blind study" to avoid bias results, so everyone will be unaware what the solution consists of.
Purpose
The purpose of my experiment was to determine how caffeine affected the heart rate of Daphnia.
Hypothesis
I believe that a Daphnia subjected to a caffeine solution will show a rise in heart rate, this rise in heart rate should reflect the concentration of caffeine in a given solution. My theory can be backed up well, as caffeine is a stimulant that speeds up the body affecting the nervous system and rate in which the nerve pulses are sent and received in humans.
Materials
Daphnia
6 Cavity slides
Dropping pipette
Distilled water
Caffeine solutions
Cotton wool
Pipettes
Test tubes
Stop clock
Paper towels or filter paper
Microscope
Method
Place a few strand of cotton wool onto the cavity slide, this will help restrict the movement of the Daphnia making it easier to see under the microscope. Using a pipette place a daphnia on the cotton wool. The microscope should not be on a high power and will be centred on the heart. The first test on the heart rate will be done with just the pond water in which they live, so we have a set of results that can compare to when they are intoxicated by the caffeine. The heart rate will be recorded at intervals of 2...
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