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Cloning

Submitted by funboy14 on April 28, 2008

Category: Social Issues
Words: 1697 | Pages: 7
Views: 112
Popularity Rank: 93,017
Average Member Grade: N/A (Add a Comment / Grade this Paper)

Cloning is the process of creating an identical copy of something. This means that every single bit of DNA is the same between the two. In biology, it collectively refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. There are different types of cloning and cloning technologies that can be used for other purposes besides producing the genetic twin of another organism. A basic understanding of the different types of cloning is key to taking an informed stance on current public policy issues and making the best possible personal decisions. The following three types of cloning technologies are: recombinant DNA technology or DNA cloning, reproductive cloning, and therapeutic cloning.

Recombinant DNA Technology or DNA Cloning

Recombinant DNA technology, or DNA cloning refers to the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Scientists studying a particular gene often use bacterial plasmids to generate multiple copies of the same gene. Plasmids are self-replicating extra-chromosomal circular DNA molecules, distinct from the normal bacterial genome. Plasmids and other types of cloning vectors are used by Human Genome Project researchers to copy genes and other pieces of chromosomes to generate enough identical material for further study. To clone a gene, a DNA fragment containing the gene of interest is isolated from chromosomal DNA using restriction enzymes and then united with a plasmid that has been cut with the same restriction enzymes. When the fragment of chromosomal DNA is joined with its cloning vector in the lab, it is called a recombinant DNA molecule.

Reproductive Cloning

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