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Biology Coursework: The effect of Trypsin on Gelatine. Implementing: - 1. First,
I gathered the necessary equipment: ง Thermostatically ...
Submitted by joehigashi on May 22, 2005
Category: Science
Words: 681 | Pages: 3
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Implementing: -
1. First, I gathered the necessary equipment:
ยง Thermostatically controlled water baths
ยง Ice
ยง Thermometer
ยง Stop watch
ยง Test tubes
ยง Photographic film (b/w)
ยง Syringe
ยง Test tube rack
ยง Ruler
ยง Scissors
ยง O.1% trypsin made in pH7 buffer solution
ยง Mounted needle
2. I then decided on the range of results and temperatures I was to investigate. I decided to observe 20ยบ, 30ยบ, 40ยบ, 50ยบ, 60ยบ, 70ยบ and 80ยบ.
3. Next, I label each test tube with the temperature to be investigated and then added 2.5cm3 of Trypsin solution.
4. I ensured that I cut the necessary pieces of film using a ruler and a pair of scissors. I cut pieces that were 2cm in length, and 1.6cm in width each.
5. The only water baths of my choice that were running were at 30ยบ and 60ยบ. I therefore had to use the Bunsen burner etc. for the rest of the temperatures (excluding 20ยบ). I kept each test tube heated for 5 minutes.
6. I added the photographic film pieces just before I placed the test tubes in the water baths/ Bunsen burners, and ensured that they submerged fully before I began timing.
7. I carefully and frequently examined each test tube for the time that the film became transparent and listed the result
Results: -
Temperature (ยบC) 20 30 40 50 60 70 80
Time taken (minutes) 23:38 16:37 6:06 6:40 7:53 8:24 10:15
Time taken (seconds) 1418 997 366 400 473 504 615
Rate of Reaction -(1/time in mins) 4.d.p 0.0426 0.0606 0.1667 0.1538 0.1250 0.1176 0.0976
It is evident from the graph and the table above, that as the temperature is increased, the amount of light passing through the tested solutions is decreased, and therefore the amount of pigment released from the beetroot samples is increased.
The results produce a graph with negative...
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